Nutrients

Dissolved inorganic nutrients

(nitrate, nitrite, ammonium, silicate, silicate) are analyzed on an AA3 Nutrient AutoAnalyzer according to methods specified by the SOEST Laboratory for Analytical Biogeochemistry (S-LAB):  http://www.soest.hawaii.edu/S-LAB/equipment/slab_autoanalyzer.htm

Total dissolved phosphorus (TDP)

Via high temperature ashing method (HTA) of Monaghan and Ruttenberg (1999)., available at S-LAB

 

Dissolved organic carbon and total dissolved nitrogen (DOC, TDN)

are analyzed on a Shimadzu High-Temperature TOC-L Combustion Analyzer according to methods specified by the SOEST Laboratory for Analytical Biogeochemistry (S-LAB):  http://www.soest.hawaii.edu/S-LAB/equipment/slab_TOC-L.htm

 


Biological activity

Chlorophyll a

Via fluorometric analysis according to methods specified by the SOEST Laboratory for Analytical Biogeochemistry (S-LAB):  http://www.soest.hawaii.edu/S-LAB/equipment/slab_fluorometer.htm

 

Photosynthetic accessory pigments

Via HPLC analysis according to Bidigare, R.R.,  Heukelem, L.V. and Trees, C.C. (2005) Analysis of Algal Pigments by HPLC, p. 565, Algal Culturing Techniques, Elsevier Academic Press.

 

Phytoplankton community composition

TBD (from Sue Brown?)

 

Alkaline phosphatase

Via the fluorometric method of Dyhrman and Ruttenberg (2006) as modified by Hull (2010)

 


Water quality

Total suspended solids

Ruttenberg lab protocol (1999)

 


Sediment

Sediment grain size distributions

TBD